First, boot

Remove the desiccant from the sample compartment before starting up and check that the power supply is connected. Turn on the power switch of the instrument, wait for the instrument to pass the self-test, and prohibit opening the sample chamber during the self-test.

Second, use

After the self-test is completed (there are OK words appearing in all seven items), the instrument enters the main menu and the screen displays the following seven functional items: 1. Photometric measurement; 2. Spectral measurement; 3. Quantitative measurement; 4. Kinetic measurement; Data processing; 6. Multi-wavelength measurement; 7. System state setting. After 30 minutes of thermal stabilization, the instrument can enter normal measurements.

1. Photometric measurement of light transmittance (T%) or absorbance at a given wavelength (A)

After selecting the [Photometry] item in the main menu, press the [ENTER] key to enter this function block → press the [GOTO WL] key to enter the wavelength setting Enter the desired wavelength value using the number keys → press the [ENTER] key to confirm → screen Tip: Please wait ..., the instrument will automatically shift the wavelength to the wavelength you want to measure → press [F1] key, select the measured transmittance (T%) or absorbance (A) → Open the sample chamber cover, Place R and S1 positions of the cuvette rack between the blank solution and the sample to be measured. Close the cover of the sample chamber. → Press the [F3] key. The instrument automatically moves the position of the cuvette holder R to the light path (ie, blank solution) on the screen. Display Cell = R → Press the [AUTO ZERO] key. The instrument automatically zeros the blank solution. Screen prompt: Please wait... → Press [F2], the cuvette moves to S1 (sample to be tested), and the screen displays Cell=S1 → Press [F4] to measure T% or A value

After the measurement is completed

1) Print out the data and press [START/STOP]

2) Return to the main menu and press the [MODE] key

2. Spectral measurement wavelength scanning or spectral scanning, which can directly measure the spectrum and peak valley data in a wavelength range

After selecting the [Spectral Measurement] item in the main menu, press the [ENTER] key to enter this function block. → Set the measurement mode, sweep range, recording range, sweep speed, sampling interval, number of sweeps, and display mode parameters as required. Press the [→], [←], [↑], [↓] arrow keys to reach the setting line, modify the parameters, and press the [ENTER] key to confirm. → Open the sample chamber cover and place the blank solution and the sample to be tested. The R and S1 positions of the cuvette holder close the sample chamber lid → Press [F3], the instrument automatically moves the position of the cuvette holder R to the light path (ie, blank solution). Cell=R is displayed on the screen. → Press [F1] ] Baseline correction. Screen prompts: Baseline correction... → Press [F2], the cuvette moves to S1 (sample to be tested), and the display shows Cell=S1 → press [START/STOP] key to start spectrum scanning → display scan pattern

After the scan

1) Print the result spectrum, press [F4]

2) Read the peak and valley value directly, press [F2] key, the screen displays “please enter the peak/valley detection sensitivity” (default is 3), press [ENTER] to confirm

3) Store the spectrum and obtain the data in the entire wavelength range. Press [F3], use the [→], [←] arrow keys to select 10 digits and 26 letters to compose the file name, press [ENTER] to confirm, press [ F4] Store, press 1-8 number keys to determine the file serial number

4) To modify the spectrum coordinates, press the [F1] key. After modifying, press [START/STOP] to confirm, and the spectrum will be refreshed according to the new set coordinates.

5) Return to the previous menu and press the [MODE] key

3. Quantitatively establish the concentration curve and directly measure the concentration of the sample

After selecting the [Quantification] item in the main menu, press the [ENTER] key to enter this function block → Set the parameters for the measurement wavelength, measurement unit, and measurement method as required. Press the [→], [←], [↑], [↓] arrow keys to reach the setting line, modify the parameters, and press the [ENTER] key to confirm

The 3.1 K-factor method has a known slope of k and intercept b. After measuring the absorbance of the sample, it can be calculated by entering the formula.

Select the K-factor method in the measurement method and press the [ENTER] key to confirm. Press the [F2] key to enter the k-value and b-value. Press the [ENTER] key to confirm. → Open the sample chamber cover. The cuvette in the current light path Put a blank sample in the rack and close the sample compartment lid → Press [AUTO ZERO] to zero → Place the sample in the rack → Press [START/STOP] to enter the data measurement interface → Press [F2] to move Cuvette holder, place the measured sample in the light path → press [START/STOP] key to measure

3.2 Single-point calibration method to measure the absorbance of a standard sample and set the working curve of the zero point, determine the unknown sample concentration

Select single point calibration method in measurement method and press [ENTER] key to confirm → press [F2] key to modify single point → press numeric key to input the concentration value of standard sample → press [ENTER] → open sample chamber lid, at current Place a blank sample in the cuvette shelf of the optical path and close the sample compartment lid → Press [AUTO ZERO] to zero → Replace the blank sample with a standard sample → Press the [START/STOP] button to measure the absorbance of the sample and display → Place the sample in the cuvette holder → Press the [START/STOP] key to enter the sample measurement interface → Press the [F2] key to move the cuvette holder so that the sample to be measured is in the light path → press the [START/STOP] key to measure

3.3 multi-point calibration method to measure the absorbance of a series of standard samples of known concentration, establish the working curve to determine the unknown concentration

Select the multi-point calibration method in the measurement method and press the [ENTER] key to confirm. → Open the sample chamber cover, place a blank sample in the cuvette shelf of the current light path, close the sample chamber cover, and press the [AUTO ZERO] key Zero → Press [F2] to recalibrate → Press number key to enter the number of standard samples → Press [ENTER] to confirm → Place the standard sample in R, S1, S2 positions in the cuvette rack... Close the sample compartment cover → press [ F3] Move the rack R to the light path → press the [ENTER] key to confirm → press the number keys to enter the standard density value, press the [ENTER] key to confirm → press the [START/STOP] key to measure the absorbance of the standard → press [ Press the [F2] key to move the sample position S1 to the light path. Measure the absorbance of all the standards in the same way. Press the [F1] key to generate the equation curve. The slope, k, intercept b, and correlation coefficient R of the curve are displayed. → Press the [MODE] key to return to the quantitative measurement menu → Press the [START/STOP] key to enter the data measurement menu → Insert the sample → Press the [F2] key to move the colorimetric cell holder so that the sample under test is in the light path → press [START/ STOP] key measurement

6. Multiwavelength determination of light transmittance (T%) or absorbance at several wavelengths simultaneously (A)

After selecting [Multiwavelength Measurement] in the main menu, press the [ENTER] key to enter this function block → Press the [F3] key to set the number of measurement wavelengths and the number of sample cells, press the [ENTER] key to confirm → press the number keys to enter the corresponding Wavelength value → Open the sample chamber cover, place the blank solution and the sample to be measured into the rack R, S1, and S2..., close the sample chamber lid → press the [START/STOP] button to start measurement

After the measurement is completed

1) Print out the data, press [F4]

2) Return to the main menu and press the [MODE] key

Third, shut down

Invert the solution in the cuvette, then rinse the cuvette with distilled water or organic solvent until it is clean and dry it upside down. Turn off the power and put the desiccant into the sample chamber, put on the dust cover, make registration, and get approval from the management teacher before leaving.

Precautions:

1. Remove the desiccant from the sample chamber before starting up. Do not open the sample chamber cover during the self-test.
2. The solution in the cuvette is preferably 2/3 to 4/5 of the dish height, and should not be overfilled to prevent the liquid from overflowing and corroding the instrument. The cuvette should be kept clean during the measurement, and the liquid droplets on the pool wall should be wiped dry with the lens tissue. Do not pinch the light surface with your hands. When measuring ultraviolet wavelengths, quartz cuvettes are used.
3. During the measurement, it is forbidden to place the reagent or liquid substance on the surface of the instrument. If there is solution overflow or other reasons, the sample tank is dirty, and it should be cleaned as soon as possible.

4. After the end of the experiment, the solution in the cuvette is depleted, and then the cuvette is washed with distilled water or an organic solvent to clean it and dry it upside down. Turn off the power and put the desiccant into the sample chamber, put on the dust cover, make registration, and get approval from the management teacher before leaving.

Problem processing:
1. If the instrument fails to initialize, shut down and restart. If it is not successful, please report to the management teacher.
2. If the absorption value is abnormal, check in sequence: whether the wavelength setting is correct (re-adjust the wavelength and re-zero), whether the measurement is zero (if mis-operated, re-zero), and whether the cuvette is used wrongly (measurement of ultraviolet In the case of a band, quartz cuvettes are used, and sample preparation is erroneous (in case of errors, prepare the sample again).

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