[ Instrument Network Instrument R & D ] King Abdullah University of Science and Technology (KAUST) was established in September 2009. It is an international and research university in Saudi Arabia with advanced scientific research equipment and laboratories in the world. The school currently does not enroll undergraduates, and only recruits graduate and doctoral students worldwide. It is understood that recently, researchers at King Abdullah University of Science and Technology (KAUST) have shown how to obtain more information through a standard microscope.
In order to promote the development of biology, the development of microscope technology is very important. The basic imaging principle of the microscope is to measure the intensity of light passing through an object to form an image. There is therefore a need for samples that can scatter and absorb light in different ways. However, it is well known that many living cells can absorb very little visible light, which means that the difference between light and dark areas is very small, which is what we commonly call contrast. It also makes it difficult for biologists to see finer details.
However, the light passing through the sample not only changes its intensity, it also changes its phase: the relative time of each peak in the light wave. Congli Wang, a doctoral student at KAUST, explains: "Phase-contrast microscopes can transform phases into larger amplitude changes, so you can observe more precise, detailed and transparent structures."
Wang and his colleagues at the KAUST Visual Computing Center, led by computer science professor Wolfgang Heidrich, have now developed a new method for quantitative phase and grayscale imaging. The device that is critical to the performance of a microscope is called a wavefront sensor. The wavefront sensor is a sensor designed for adaptive optics, which encodes the information of the wavefront phase into a grayscale image.
Researchers use quantitative phase imaging to detect the geometry of transparent small samples with fine structures; for example, three-dimensional red blood cells (left) and microlens arrays (right).
The team has designed an innovative high-resolution wavefront sensor that is currently being integrated into commercial microscopes to improve the performance of microscope imaging. At the same time, they also reconstructed the phase contrast image through a computer algorithm developed by themselves, and digitally retrieved the quantitative phase from a pair of images: the calibration image obtained when there is no sample and the measurement image obtained when the sample is in place.
This method simplifies the use of the microscope in several ways. Although some methods have achieved quantitative phase imaging in the past, they are costly and complex to set up and require a dedicated light source or a long time to generate the image. "Our method enables fast calculations with simple, inexpensive optics, ordinary white light sources, and real-time video transmission rates. Snapshots capture high-resolution bright-field amplitudes and accurate quantitative phase images." Heidrich said, You know, it's a combination of all these advantages. "
A microscope is an optical instrument composed of a lens or a combination of several lenses, and it is a sign that humans have entered the atomic age. It is mainly used to magnify tiny objects into the human eye. Imaging is the imaging technique of biological samples. According to the size of the sample, it can be roughly divided into tissue imaging and cell molecule microscopy. These generally require the development of optical technology to match the characteristics of biological samples. A few will use wave properties other than light, such as nuclear magnetic resonance, ultrasound, and so on.
Source: Mymes Consulting, Baidu Encyclopedia
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