Atomic absorption spectrometer principle and composition

Atomic Absorption Spectrometer with high sensitivity (up to 10-1 ~ 10g / L) repeatability and selectivity is good. Easy to operate and fast. The result is correct and reliable. The amount of sample used during the test is small (between a few microliters to ten microliters in children), and the advantages of measuring a wide range of applications (almost for analyzing all metal elements and metalloid elements) can be obtained. It can be used in many fields such as metallurgy, chemical industry, geology, agriculture, medicine and health; it also has wide applications in environmental monitoring, food hygiene, and the determination of trace metal elements in biological organisms, as well as medical and biochemical tests.

The body contains many metallic elements that are important for maintaining normal physiological processes, such as potassium, sodium, calcium, magnesium, iron, copper, zinc, manganese, molybdenum, and cobalt. Human blood, sweat, urine, hair and body tissues. Due to environmental and dietary pollution, harmful elements such as lead, mercury, cadmium, and arsenic can be introduced into the body.分析 The analysis results of these metal elements can reflect the physiological processes in the body and the situation of poisoning by environmental pollution. Atomic Absorption Spectrometry can be used to analyze trace elements in blood, urine, feces and biological tissues. The viscera, hair, bones, etc. can also be analyzed and determined after a certain treatment. The basic principles of the 1 atomic absorption spectrometry method in nature. All material molecules are made up of atoms, and the original is composed of a nuclear and extranuclear electrons. There are neutrons and protons in the nucleus and the protons are positively charged. The external nuclear charge is negatively charged; the number of days and configuration of its electrons determine the physical and chemical properties of the element. Electricity rotates around the nucleus according to a certain orbit; according to the distance from the nucleus of the electron orbit, there are different energy levels and can be divided into different shells. The number of electrons promised by each shell is certain. When the atom is in normal condition. Each electron tends to occupy a low energy level, and the state of the atom is called the ground state (E0). Under the action of heat energy, electric energy or light energy, electrons in atoms absorb certain energy. Electrons in a low energy state are excited to transition to a higher energy state. The state of the atom at this time is called the excited state (Eq). The process of the transition of atoms from the ground state to the excited state is the process of energy absorption. The atoms in the excited state are unstable and generally return to the ground state (E0) or the lower excited state (Ep) within 10-10~-10-8s. At this point, the atoms release excess energy and radiate out the photon beam. The amount of radiant energy is expressed by the following formula:

AE=Eq-Ep (or E0)=hf=hc/λ(1)

Where: h--planck constant is 6.6234x10-27 erg.s; the frequency and wavelength of the spectrum emitted by the f and λ-electrons when returned from the Eq level to the Ep (or E0) level; C-- Light speed.

Eq, Ep, or E0. The size of the value is related to the atomic structure, different elements, Eq, Ep, and E0. Differently, the general element atoms can only be emitted by their EqEp or Eo. The specific wavelength or frequency of light, ie:

f=Eq. One Ep (or E0)/h(2)

The atoms of each substance have a specific atomic structure and an outer electron arrangement, so that different atoms are excited. Its electrons have different transitions. Can radiate different wavelengths of light, that is to say. Each element has its own spectral line. Since the intensity of the spectral line is proportional to the content of the element, the content of the element can be determined.

For quantitative analysis.

When an element is excited, the extranuclear charge is excited from the ground state E to the lowest excited state E1 that is closest to the ground state. When its fork returns to E. The emitted radiation is its vibration line. The ground state atomic absorption resonance radiation can also rise from the ground state to the lowest excited state, because the resonance lines of various elements are not the same, and has certain characteristics. Therefore, the atomic absorption spectrometer can be observed in certain characteristic wavelengths of the same element. When light emitted by a light source of a certain characteristic wavelength passes through the original vapor of the sample to be measured. The outer electrons in the atom will selectively absorb the characteristic lines emitted by the same element, so that the incident light emitted by the light source will be weakened, and the degree of attenuation of the characteristic spectrum due to absorption will be expressed by the absorbance A, A and measured. The element to be measured in the sample contains the most direct proportion; that is, the greater the concentration of the ground state atoms, the greater the amount of light absorbed. By measuring the amount of light absorbed, the halo of the metal and the metalloid species to be measured in the sample can be determined. For most metal elements, the resonance line is the most sensitive of all the lines of the element.

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